preparation of fibrin glue as a biological sealant to control bleeding in heart muscle and blood vessels

fibrin glue is used for control of bleeding in various surgical procedures. in this work the ability of fibrin glue to seal punctures in the vascular system is demonstrated. blood samples were taken from rabbits, fibrinogen was separated and fibrin glue was eventually prepared. the rabbits were anesthetized and a midline incision was carried out. the heart and abdominal aorta were exposed. punctures were made in different parts of the vascular system and bleeding was controlled either with (test) or without (control) fibrin glue. oozing was also tested by scratching the rabbit's ears. a minimum of 6 rabbits was employed for each experience. the mean bleeding time using fibrin glue was found to be 37 seconds. this average without fibrin glue was more than 3 minutes. this study shows the powerful effect of this biological glue in bleeding control, and its routine use is therefore recommended, especially in major surgery.